ORIGINAL PAPER Bacterial contamination of amniotic membrane in a tissue bank from Iran
ORIGINAL PAPER Bacterial contamination of amniotic membrane in a tissue bank from Iran

ORIGINAL PAPER Bacterial contamination of amniotic membrane in a tissue bank from Iran

AMs that is produced by HPT for several ophthalmic applications.  From  July  2006  to  April  2011,  122 placentas  from  cesarean  sections  were  retrieved  by HPT   after   obtaining   informed   consent   from   the donors.  Besides  testing  donor’s  blood  sample  for viral  markers,  microbiological  evaluation  was  per- formed   pre   and   post   processing.   During   tissue processing,  decontamination  was  performed  by  an antibiotic  cocktail  including;  Gentamicin,  Ceftriax- one  and  Cloxacillin.  Of  271  cesarean  section  AM donors  who  were  screened  as  potential  donors,  122 were   accepted   for   processing   and   assessed   for microbiological   contamination.   Donors’   age   were between  21  and  41 years  (Mean = 27.61 ± 0.24). More  than  92 %  of  mothers  were  in  their  first  or second  gravidity  with  full  term  pregnancies.  The most prevalent organisms were Staphylococci species (72.53 %).   After   processing,   contamination   rates markedly  decreased  by  84.62 %  ( p value = 0.013). According  to  our  results,  most  of  bacterial  contam- inations  were  related  to  donation  process  and  the contamination pattern suggests procurement team as a   source.   Therefore   we   recommend   that   regular training  programs  should  be  implemented  by  tissue banks for procurement staff. These programs should focus   on   improved   donor   screening   and   proper aseptic technique for tissue retrieval. We also suggest that  tissue  banks  should  periodically  check  the  rate and  types  of  tissue  contaminations.  These  data  help them  to  find  system  faults  and  to  update  processing methods.

منبع:

http://www.baradaran-rafii.com

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